Apimondia 2015 Abstract book (South Korea)

ATP-012

Antiurolithiatic Effects of hydro-alcoholic extract of propolis on Ethylene-Glycol-Induced Nephrolithiasis in Rats

Badiaa Lyoussi, Nawal El Menyiy, Meryem Bakour

Laboratory Physiology-Pharmacology & Environmental Health, Faculty of Sciences DHM, University

Sidi Mohamed Ben Abdallah, Fez, Morocco

Propolis is a natural honeybee product known to be beneficial for human health, with a complex chemical composition, highly dependent on the collection site. The aim of our study is to evaluate the preventive effect of hydro-alcoholic extract of propolis in ethylene-glycol induced urolithiasis in the male Wistar rats. Materials and methods: Wistar albino rats were given 0.75% v/v ethylene glycol in drinking water to induce chronic hyperoxaluria and simultaneously hydro-alcoholic extract was given to nephrolithiasic treated rats at the dose of 100 and 250 mg/kg b.w. orally for 30 days, Cystone (500 mg/kg, p.o.) served as a standard. The weight difference, pH, Urinary volume, urinary crystals and the levels of calcium, magnesium, phosphorus, sodium, potassium, chloride, proteins, creatinine and uric acid in 24h-urine were measured. The biochemical parameters and the transaminase (alanine aminotransferase, ALT; aspartate aminotransferase, AST), phosphatase alkaline activity (PAL) were also evaluated in both serum. Results: The results show that the EG treatment lead to increased pH, urine volume and the levels of calcium, phosphorus, uric acid and protein in urine compared to healthy rats. The decrease in the magnesium, creatinine, sodium, potassium and chloride. Levels were normalized by hydroalcoholic extract of propolis and Cystone treatments. Moreover, the biochemical parameters were also largely normalized by the extract, which could contribute to the antiurolithiatic property of the extract. Conclusions: The results of this study indicate very promising antiurolithiatic effect of the hydro-alcoholic extract of propolis with preventive treatments in this experimental condition

ATP-013

Protective effect of Moroccan Carob honey against carbon tetrachloride (CCl4) induced liver injury in rat

Badiaa Lyoussi1, Redouan El-haskoury1, Zineb Kamoun2, Mohamed Makni2

1 Laboratory of Physiology, Pharmacology and Environmental Health, Faculty of Sciences Dhar El Mehraz, BP 1796 Atlas, University Sidi, Mohamed Ben Abdallah, Fez 30 000, Morocco.

2 Toxicology-Microbiology and Environmental Health Unit (UR11ES70), Faculty of Sciences, University of

Sfax, Tunisia.

Honey contains various biological active substances including proteins, free amino acids, enzymes, vitamins, organic acids, flavonoids, phenolic acids and other phytochemicals. Honey has antioxidant, antiviral, antimicrobial, antiparasitic, and antimutagenic properties. This might explain its wide range of the therapeutic and biological activities. The present study was aimed to investigate the hepatoprotective effect of carob honey on carbon tetrachloride (CCl4) induced liver injury. The Wistar rats were used for the study and the hepatotoxicity was induced by intraperitoneal injection of CCl4 (1ml/kh b.w.) after four days of pretreatment with carob honey

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(2g/kg b.w. : orally). The hepatoprotective effect of carob honey was determined by assessing the biochemical parameters in serum and the markers of oxidative stress in liver. Pretreatment with honey prior the administration of CCl4 significantly prevented the release of transaminases, alkaline phosphatase, lactate dehydrogenase, significantly inhibited lipid peroxidation (MDA) , protein carbonyl (PCO) formation and Advanced protein oxidation products (AOPP) in liver and markedly enhanced catalase , glutathione peroxidase and ascorbic acid in liver. This findings confirmed the hepatoprotective effect of carob honey against CCl4 toxicity.

ATP-014

Determination of flavonoid classes in Trigona sp. propolis from Luwu regency, South Sulawesi province, Indonesia

Sherly Horax1, Ardo Sabir1, Latief Mooduto2, Cahyono Kaelan3

1 Faculty of Dentistry, Hasanuddin University

2 Faculty of Dentistry, Airlangga University

3 Faculty of Medicine, Hasanuddin University, Indonesia

Propolis is a resinous hive product, collected by bees. Recently, propolis has been used clinically by dentist to treat various oral diseases such as to support aid for the healing of post-extraction wounds, as an antimicrobial agent in the treatment of dental caries and pulpal infections, and to promote dentinal bridge formation in direct pulp-capping treatment. Propolis contains waxes, ferulic acid, cinnamic acid, phenol acids, aromatic aldehydes, and a large number of flavonoids. The application of propolis preparations in dentistry leads to increase interest in its chemical composition.

The aim of this research was to determine flavonoid classes in Trigona sp propolis which was collected from honeycombs in Luwu regency, South Sulawesi Province, Indonesia. Propolis was subjected to exhaustive maseration, filtered using aqueous ethanol solution and concentrated using a rotary evaporator. The residue was separated using toluene solution to yield polar flavonoid fraction and semi-polar flavonoid fraction which was analyzed using Thin Layer Chromatography (TLC) method (silica gel GF254 precoated plates with n-butanol: acetic acid: water (3: 1: 1 v/v) as mobile phase). The purification of flavonoids was carried-out by UV-radiation at 2 5 3 nm and 366 nm and treatment with ammonia. The idenfication flavonoid classes based on color reactions according Mabry et al.

The result of this research showed that the flavonoid classes in the Trigona sp propolis from Luwu regency, South Sulawesi province, Indonesia were flavones, flavanone, flavonol, and chalcone classes.

ATP-015

Apimanaxx - honey from uncaria tomentosa (willd.) Dc: A new organic functional product that protects natural immunity and prevents premature radiation aging. Results of clinical studies

Viacheslav Tsuprykov

461

Environmental NGO

New honey with brandname APIMANAXX, containing active substances from Uncaria tomentosa (Willd.) DC. or Uña de Gato or Cat's Claw - legendary wild liana from Amazon rain forest or selva del Peru, a herb which has historical usage for the treatment of several human disorders including cancer, inflammatory and infectious diseases, was first obtained in apiary "Gogolmed" in Orzhitsky district, Poltava region, Ukraine.

APIMANAXX was tested on efficacy and safety in National Research Centre for Radiation Medicine of NAMS. In clinical study were attended 42 volunteers from number of clean-up workers of Chernobyl disaster. Every day they have ate 20 grams of honey for 28 days. As a placebo (control group was formed from 20 subjects) was taken an acacia honey that was the source-honey for APIMANAXX production. Before taking honey and after its completion, each participant of clinical study was a subject of laboratory examination (clinical, biochemical, immunological blood tests, general urinalysis, ECG, encephalogram), psychological tests (verbal, cognitive, intellectual). It was found that APIMANAXX is completely safe and has positive impact on human body. Laboratory investigations showed stimulation of leuko-and erythropoiesis, an increase of hemoglobin levels and platelet count, increasing numbers of cytotoxic T- lymphocytes, decreased titers of antiviral antibodies. Studies of radiation parameters of cellular aging demonstrated the stabilizing effect of honey on a relative telomere length and entry of cells into apoptosis.We have examined APIMANAXX for the presence of Carboxy alkyl esters and have found that this compounds are responsable for it immune-enhancing properties.

ATP-016

Antibacterial activity of bee venom

Ahmed Hegazi

National Research Center

Bee venom is very complex mixture of active peptides, enzymes, and amines. The composition of the venom produced by the glands of Apis mellifera has been well documented. The therapeutic application of bee venom, has been used in traditional medicine to treat diseases. It has biological activity as inhibit mammary carcinoma cell proliferation), cytotoxic to maligna nt cells both in vitro, arthritis, rheumatism, pain, cancerous tumors, and skin diseases, rheumatoid arthritis and osteoarthritis. Honey bee (Apis mellifera) venom therapy (apitherapy) has been elucidated therapeutic value for bacterial diseases and reported to be as effective as antibacterial drugs. Antimicrobial activity on some Gram-negative bacteria as Escherichia coli and Salmonella spp., Enterobacter cloacae, Escherichia coli and Citrobacter freundii and Staphylococcus aureus, Coagulase-negative Staphylococcus and E. coli. The aim of this review was, therefore, to evaluate the data from antimicrobial activity of bee venom.

ATP-017

Multiple Sclerosis patients treated with Apitherapy: Immunological pattern

Ahmed Hegazi

National Research Center

Objective: Determination of immunological pattern of patients suffering from multiple sclerosis treated with apitherapy in particular using bee sting therapy. Methods: Fifty patients with MS, their ages ranged between 26-71 years, were subjected to complete clinical and neurological history and examination to confirm the

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diagnosis. All cases were under their regular treatment they were divided into two main groups, Group I received honey, pollen, royal jelly and propolis and were treated with apiacupuncture 3 times weekly, for 12 months, in addition to their medical treatment, while group II remains on their ordinary medical treatment only. IFN-, interleukin (IL ) 1, IL-4, IL-6, IL-10, tumor necrosis factor alpha (TNF) were detected. Apiacupuncture was done by bee stings for regulating the immune system. Results: Results revealed that 4 patients showed some improvement regarding their defects in gait, bowel control, constipation and urination, while 12 cases, showed some mild improvement in their movement in bed, and better improvement in bed sores, sensation, and better motor power, only two cases of them were able to stand for few minutes with support. The level of IFN-, (IL) 1, IL-4, IL-10, TNFwas significantly elevated in patients in Group II, and no significant differences were found for IL-6 between the 2 groups of treatment. The mean values of IgE level in both groups of M.S. patients were low but with no statistical significance, while by the end of the study there were an elevation in the levels of IgE for both groups which was statistically significant.

ATP-018

Influence of royal jelly, ubiquinone and their composition on balance of pro-and antioxidant systems in the blood of rats with heat injury

Vasily Krylov, Elena Krylova, Anna Solovieva, Alina Chaplygina

Lobachevsky State University of Nizhny Novgorod, Russia

Experiments were carried out on 50 white rats Wistar male rats m=250-300g in 5 groups: intact; verification (thermal burn, 7sec); and 3 advanced-animals with the burn, which daily orally injected Royal Jelly (RJ) in a dose of 100 mg/kg; Ubiquinone-10 (Q-10) at a dose of 15 mg/kg; and RJ and Q-10 together. In the 10 days after the burn in plasma and erythrocytes studied the level of the lipid peroxidation (LP) and the overall antioxidant activity (AOA). Compared with healthy rats, for burns in plasma increased level of LP and number of Malonic Dialdehide (MDA) at 5% and 45%, respectively. Plasma AOA decreased on 13% and activity of Superoxyddismutase (SOD) decreased in 31%. Application of postburn RJ reduces the intensity of LP, the increase of SOD activity at 91%. Number of MDA has decreased in comparison with control and healthy animals at 50% and 28%, respectively. Introduction of Q-10 has increased the plasma AOA and activity of SOD in 31% and 29%, respectively, and the content of MDA in plasma by 58%, compared to the animals with the burn. Introduction of "RJ + Q1-10" reduced the rate LP and MDA in plasma by 11% and 44, respectively, increased the activity of SOD at 87%, compared to the controls. In erythrocytes membrane resistance increased, free radical oxidation they decreased by 16%. The most positive effect found when Ubiquinone-10 and Royal Jelly.

ATP-019

Application of the mixture for inhalation based on propolis and royal jelly "Apingalin" in the treatment of broncho-pulmonary diseases of various origins

Vasily Krylov, Svetlana Kopylova, Olga Cherkunova, Marina Rodavskaya

463

Lobachevsky State University of Nizhny Novgorod, Russia

Experiments were performed on 100 male rats weighing 200-250 g. There were two experimental series: pulmonary edema with modeling and experimental model tracheitis. In each of the experiments, the animals were divided into groups: intact, control (swelling of the lungs or trachea), experienced (treatment of a mixture for inhalation "Apingalin"). Experiments have shown that inhalation of "Apingalin" in a simulation tracheitis increases the total number of erythrocytes, increases the hemoglobin content, contributed to the restoration of the functional usefulness of erythrocytes and normalization of rheological properties of blood and the ratio of phospholipid fractions of erythrocyte membranes. Course inhalation means "Apingalin" when modeling adrenal pulmonary edema in rats helped reduce the intensity of the formation of endogenous intoxication: a decrease in endogenous release of toxins into the bloodstream from the lung tissue, reduced lipid peroxidation, Recovery biotransporta endogenous toxins, albumin concentration, very low density lipoproteins and low density lipoprotein; increasing the concentration of the total protein and fibrinogen and the normalization ratio protein fractions. Thus, for the treatment of broncho-pulmonary diseases can be recommended to use a mixture based on inhalation of propolis and royal jelly ”Apingalin”.

ATP-020

Efficiency of apitherapy by treatment of patients with chronic cerebral ischemia and seringomieliey

Vasily Krylov, Anna Deriugina, Elena Antipenko

Lobachevsky State University of Nizhny Novgorod

The aim of the research was to study the efficiency of apitherapy in combination with standard therapy in patients with chronic cerebral ischemia (CCI) stage II and seringomieliey (M). 50 patients with a CCI stage II, and 25 patients with a M were obser ved. Patients each disease were divided into two groups (main and control). The treatment course in the main group contained the ba sic therapy and apipuncture. Sterile solution of bee venom were injected intramuscular about 0.2 ml (single dose - 0.1 mg). The control group reseived standard therapy only. Blood counts (electrophoretic erythrocytes mobility, the concentration them in malondialdehyde and catalase activity) are examined patients of both pathologies. The dynamics of the neurological syndrome was evaluated in patients CCI and pain syndrom was evaluated in patients M. It is shown that the inclusion of apitherapy in a complex of basic therapy, more significantly improves the number of neurological indicators, pain and indicators of red blood compared with standard therapy. Apitherapy causes reduction of blood parameters to the physiological norm for about pathologies. Patients with CCI identified significant improvements dynamics cephalgic (p =0.04), cochlear (p = 0.01) and vestibular-atactic (p = 0.04) syndromes from the control group. If CM noted positive dynamics in relation to the intensity of pain on a visual analog scale (p = 0.03). Possibly, the positive influence of apipuncture in patients with CCI stage II and CM were associated with activating effect of bee venom on the adaptive mechanisms of an organism.

ATP-021

Influence of bee venom and hyperthermia on the energy metabolism of tumor-animals

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Krylov Vasily , Shabalin Mikhail, Krasnikova Olga

Lobachevsky State University of Nizhny Novgorod, Russia

Experiments are executed on white nonlinear rats females weighing 200±50g in 5 groups with 5 rats in every group: intact group; control (tumor-animals) at introduction of 1ml physiological solution; skilled 1- tumoranimals subjected to a hyperthermia 42,5C against effect of bee venom; skilled 2 - tumor-animals subjected to a hyperthermia 43,5C against effect of bee venom; skilled 3 – the tumor-animals subjected to a hyperthermia 44,5 C against effect of bee venom. Blood was taken from a hypoglossal vein. Blood plasma was exposed to research. Dynamics of change of the ATP, ADP, AMP, glucose content in blood of experimental animals was carried out by means of a blood plasma IK-spectroscopy method. It was registered ranges in the range of 1170-1025cm-1. The received results have showed a decrease in concentration of AMP, ADP, glucose and increase of the ATP level at experimental animals in relation to control at a hyperthermia (42,5C; 43,5C) against effect of bee venom 1 days later after therapy ( 0.05). The hyperthermia at 42,5C causes decrease in ADP and glucose concentration since the 7th days after therapy, however the hyperthermia of 43,5C doesn't cause their change. At 44,5C all contents increases authentically ( 0.05). For the 28th days there is an increase in the AMP, ADP level, and decrease in ATP and glucose level in all skilled groups ( 0.05).

ATP-022

Pharmaceutical Activities of Darae (Actinidia arguta) pollen

In Pyo Hong, Soon Ok Woo, Sang Mi Han

National Academy of Agriculture and Science, Republic of Korea

Bee pollen is male reproductive cells of plants, which are made up of natural flower pollen by worker honeybees into granules with added honey or nectar. The incredible nutritional and medicinal value of bee pollen has known for thousands of years. It contains most of the known nutrients such as sugars, proteins, lipids, vitamins and flavonoids. Pollens have been extensively used in functional food, folk medicine, and beverage industry to improve human health. Recently, Bee pollen is receiving special attention to the potential therapeutic benefits field; bee pollen can plays a positive role in promoting the growth of endocrine glands and enhancing their secretionary functions. However stiff pollen wall hinder dissolution of polysaccharides and lower extraction efficiency. This study was conducted to establish the optimized protocol for cytoplasm isolation of bee pollen. We measured the antioxidant activities as DPPH radical scavenging, the total polyphenol content and NO production of the pulverized and lyophilized darae pollen to confirm the husk removal effect.

ATP-023

Improvement storability of the fresh-cut vegetables with propolis

Soon Ok Woo, Sang Mi Han, In Pyo Hong

National Academy of Agriculture and Science, Republic of Korea

465

Propolis is a health food, known that high antioxidant and antimicrobial effects, fresh cut vegetables that rapidly increasing consumption has recently faced the problem storability fall down after washing. To improve storability of fresh cut vegetables are being carried out various studies. In this study, using the characteristics of propolis we were performed to improve the storability of fresh cut vegetables. There was prepared in 18% solution of propolis extracts, by using this solution, propolis solution prepared diluting 0.001 to 1%, were dipped in fresh vegetables(cabbage lettuce, perilla leaf, and lettuce). Vegetables were measured the sensory evaluation and hardness after each treatment by placing in a certain period of time at room temperature and refrigerator. The results showed that the storage stability is excellent compared to non-treated as if diluted to 0.1-0.01% propolis solution is to improve the shelf life of fresh cut vegetables.

ATP-024

Antimicrobial efficacy of mastoparans against multidrugresistant Acinetobacter baumannii and Escherichia coli clinical isolates

Chun-Hsien Lin1, Chuen-Fu Lin2, Mong-Chuan Lee , Wu-Chun Tu1

1 National Chung Hsing University

2 National Chiayi University

3 Central Taiwan University of Science and Technology

Emergence of multidrug-resistant pathogenic bacteria, e.g., Acinetobacter baumannii and Escherichia coli, has become serious problems worldwide and it limited therapeutic options to combat bacterial infection. Therefore, it is an urgent need to seek new antimicrobial agents from natural resources, e.g., mastoparans (MPs) isolated from hornet venoms or to develop alternative therapy, e.g., antimicrobial combination. MPs, cationic tetradecapeptides, exhibited potent antimicrobial activity against both Gram-positive and -negative bacteria, especially Acinetobacter baumannii and Escherichia coli. In this study, it found that mastoparanAF (MP-AF), isolated from the venom of Vespa affinis, showed superior activity against multidrugresistant Acinetobacter baumannii (MDRAB) as compared with ampicillin, cephalothin, ciprofloxacin, gentamicin, neomycin, trimethoprim/sulfamethoxazole (SXT) and tetracycline. In addition, it also revealed that MP-AF exhibited synergistic activity against MDRAB when combined with ciprofloxacin or SXT. Meanwhile, mastoparan-B (MP-B), isolated from the venom of Vespa basalis, was modified to improve its antimicrobial activity by substituting amino acid content. After appropriate amino acid substitution, it found that [Leu3]-MP-B had better antimicrobial activity against multidrug-resistant E. coli and caused membrane permeabilization in a concentrationdependent manner. When [Leu3]-MP-B combined with specific antibiotic such as ampicillin, gentamicin or tetracycline, they also acted synergistically against multidrug-resistant E. coli. In conclusion, it revealed that MPAF alone or combining with specific antibiotic could display potent antimicrobial activity against MDRAB. In addition, antimicrobial activity of MP-B could be improved by appropriate amino acid substitution. Vespid venom peptides, such as MPAF or MP-B, could be promising candidates to be developed as antimicrobial therapeutics against MDR bacterial infection.

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ATP-025

Occurrence of radio resistance at rats at intragastric bee venom introduction in structure nanopartical gold

Maria Talamanova, Alexandr Koryagin, Vasili Krylov

Lobachevsky State University of Nizhny Novgorod

The work purpose – to investigate radio protective action nanostructured a biological bee venom at preventive introduction white rats-males. To skilled group entered been venom (0,5mg/kg) in structure nanoparticals gold (0,5mg/kg) and chitosan (100mg/kg), control group - a solution nanoparticals gold and chitosan. Indicators were accepted to relative norm of intact animals. All preparations were entered of 1ml intragastric of 1 times a day. Upon termination of feeding rats, control and skilled groups were exposed to the general gamma irradiation in a dose of 5Gr. The Blood sampling carried out for 1 and 28 days after an irradiation and defined total of leukocytes (Le) and quantity lymphocytes in formula of leukocytes (Lymph). During experiment it has been shown that for 1 days after irradiation Le and Lymph in group «the control on an irradiation» decreases in 4,4 and 3 times in relation to of intact animals (testifies to damaging action of gamma irradiation on blood system) and maintenance of Le in skilled group above ( <0,05) indicators of animals of group «the control on an irradiation». By 28 days total of Le group reached level of intact animals and it was above ( <0,05) indicators of group «the control on an irradiation». The quantity Limph skilled group reached level of intact animals. From the received results follows that course per os introduction to rats of bee venom in structure nanopartical led to increase of stability of animals to action of the damaging factor and radio resistance occurrence.

ATP-026

The role of propolis as a natural product in conservative dentistry and endodontic treatment

Ardo Sabir

Faculty of Dentistry, Hasanuddin University Indonesia

Propolis, or bee glue, is a natural wax-like resinous produced by honey bee from substances collected from parts of plants, buds, and exudates. Chemically, it contains waxes, ferulic acid, cinnamic acid, phenol acids, amino acids, aromatic aldehydes, and a large number of flavonoids. Propolis well known has antibacterial, anti fungal, anti-inflammatory, immunostimulator, and anti oxidant properties. Nowadays, propolis was found in many health stores in different forms like capsules, mouthwash solution, creams, toothpaste, and powder. Propolis has been used clinically by dentist to treat various oral diseases such as to support aid for the healing of post-extraction wounds, as an antimicrobial agent in the treatment of dental caries and pulpal infections, as a storage media following teeth avulsion, as a mouth rinse, and to promote dentinal bridge formation in direct pulp-capping treatment. This article is an attempt to review various applications of propolis in Conservative Dentistry and Endodontic treatment to stimulate the interest of clinicians to make further research about this natural compound.

Keywords: Propolis, Conservative Dentistry, Endodontic, Oral Health.

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ATP-027

Determination of flavonoid classes in Trigona sp propolis from Luwu regency, south Sulawesi province, Indonesia

Ardo Sabir1, Latief Mooduto2, Cahyono Kaelan3, Sherly Horax1

Propolis is a resinous hive product, collected by bees. Recently, propolis has been used clinically by dentist to treat various oral diseases such as to support aid for the healing of post-extraction wounds, as an antimicrobial agent in the treatment of dental caries and pulpal infections, and to promote dentinal bridge formation in direct pulp-capping treatment. Propolis contains waxes, ferulic acid, cinnamic acid, phenol acids, aromatic aldehydes, and a large number of flavonoids. The application of propolis preparations in dentistry leads to increase interest in its chemical composition. The aim of this research was to determine flavonoid classes in Trigona sp propolis which was collected from honeycombs in Luwu regency, South Sulawesi Province, Indonesia. Propolis was subjected to exhaustive maseration, filtered using aqueous ethanol solution and concentrated using a rotary evaporator. The residue was separated using toluene solution to yield polar flavonoid fraction and semi-polar flavonoid fraction which was analyzed using Thin Layer Chromatography (TLC) method (silica gel GF254 precoated plates with n-butanol: acetic acid: water (3: 1: 1 v/v) as mobile phase). The purification of flavonoids was carried-out by UV-radiation at 253 nm and 366 nm and treatment with ammonia. The idenfication flavonoid classes based on color reactions according Mabry et al. The result of this research showed that the flavonoid classes in the Trigona sp propolis from Luwu regency, South Sulawesi province, Indonesia were flavones, flavanone, flavonol, and chalcone classes.

ATP-028

Cardol, isolated from Trigona incisa stingless bee propolis enhances apoptotic cell death by stimulating caspase pathway in human colorectal cancer cell lines

Paula Mariana Kustiawan1, Enos Tangke Arung1, Chanpen Chanchao2, Preecha

Phuwapraisirisan3, Kriengsak Lirdprapamongkol4

1 Laboratory of Forest Product Technology, Faculty of Forestry, Mulawarman University, Samarinda, 7512

3, Indonesia

2 Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 103

30, Thailand

3 Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 1

0330, Thailand

4 Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand

Attention in the field of drug discovery has been focused on the cancer prevention of natural compounds from traditional medicinal herbs. We had demonstrated that cardol, isolated and characterized from Indonesian stingless bee propolis, induced apoptosis in human colon cancer rapidly. The apoptosis might have been through a mitochondrial and caspase-dependent pathway. Cardol, isolated from Trigona incisa propolis inhibited human colon cancer cell lines (SW620) with IC50 4.5 µg/ml and cytotoxicity in a time dependent

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manner. According to the previous result of cell apoptotic activity rapidly after treated with cardol, we evaluated anticancer mechanism of that compound and characterized the promising targets. Human colorectal cancer cell line SW620 and cell viability was determined using MTT assay. Intracellular reactive oxygen species (ROS) were assessed by DHE assay. The expression of apoptosis-associated proteins was determined by Western blotting analysis and caspase luminace analysis. Cardol in high concentration (14 µg/ml) induced apoptosis of SW620 cells, increased the expression of PARP, caspase 3 and caspase 9 and subsequent activation of caspase-3. Cardol produced intracellular ROS in a time dependent, however, N- acetylcysteine (NAC) pretreatment resulted in the inhibition of this effect and the recovery of cell viability. The activation of caspase-9, caspase-3, and concomitant poly ADP-ribosyl polymerase (PARP) cleavage, which are the indicators of caspasedependent apoptosis. This study showed that cardol exhibited a potential antitumor effect in SW620 colon cancer cells through induction of apoptosis which mediated by superoxide generation.

Keywords: cardol; stingless bee propolis; human colon cancer; apoptosis; caspase-3; ROS

ATP-029

The therapeutic efficacy and necessity of apifito composition "vitalar" for

athletes and children of Ukraine

Petro Vitenko, Andriy Zakhariah, Halina Davydova, Svitlana Hotska1, Olga Galatiuk

NSC P.I. Prokopovych Beekeeping Institute, Ukraine

We have developed a high-tech apifito dietary supplement composition "Vitalar" (homogenate of bee queen larvae on the base of honey), which proved itself as a safe and high effective supplement, that should be included in the training practice of highly skilled athletes. "Vitalar" has an indirect positive effect on the performance of structural and functional state of erythrocyte membranes, primarily by increasing the degree of antioxidant protection, indicating on the compensatory growth of the athletes organism.

There are a wide range of positive effects from dietary apifito compositions supplements. It’s easy to use and has several advantages over medical prevention means of chemical synthesis because it does not have undesirable effects even after prolonged use.

"Vitalar" is a product of high biological value, which is made of substances which are identical in structure to the natural and can act as endogenous regulators of energy processes metabolism in the body. It has such active ingredients as natural honey and royal homogenate that activate immune defenses, accelerate adaptive changes in the body and stimulate physical and mental performance.

Considering our previous positive experience with apifito compositions in the treatment of children affected by the Chernobyl accident, our objective was to investigate the therapeutic efficacy of "Vitalar" and receive a positive impact on the health of children with diseases of the gastrointestinal tract, nervous and cardiovascular systems.

So "Vitalar" is an effective cure in the treatment of children’s nervous, cardiovascular and the digestive system disorders.

ATP-030

Antimicrobial activity of korean propolis extracts against skin pathogen

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