Apimondia 2015 Abstract book (South Korea)

Propolis or honeybee glue is prepared by honeybee through combination resin or gum with wax. Due to its numerous pharmaceutical characteristic, this material is increasing considered such that it global trade has provided tremendous in co mes for the countries enjoying beekeeping industry like China, Brazil, Australia and Ukraine. Each honey bee colony produces between 50 to 300 g propolis/year. Its precise amount depends on the honey bee race, available plants and trees, climate and collection produce. World annual production is estimated to be about 300 – 400 tons. Heavy metals like lead, mercury, and arsenic that at certain level cause toxicity of the body are amongst standard factors for most countries. Permissible level of all three elements has been determined to be less than 2 ppm. Most of the Iranian propolis is produced in the northern strip of the country in the where plant sources producing propolis are abundant and from the race viewpoint as well sees are close to the Caucasian race. In order to study the level of heavy metals in the Iranian propolis, 50 samples were collected from various regions and were tested from the viewpoint of heavy metal level the amounts of which were at and even less than the standard level.

TQP-041

Late split colonies Apis mellfera using queens cells in the north of Argentina

Ee Figini5, Pa Chipulina1, Ar Lorenzo2, A Gómez3, G Schaab4

1 Á rea Apícola Ministerio de Producción – Chaco – PROAPI. Argentina chipulinapablo@hotmail.com 2 Ministerio de agricultura Ganadería y Pesca

3 Apicultor Programa Cambio Rural

4 Apicultor Programa Cambio Rural.

5 INTA. PROAPI. Fac. de Cs. Veterinarias (UNCPBA) -Tandil-Argentina

The Argentine beekeeper’s often split the hive after ended the honey flow. However the results are variable and winter survival they have been low. The objective of this assay was to assess the productive efficiency of the split of hives of end of season with the use of queen cells, in subtropical climate with AHB in the Chaco province. In an apiary of 44 Langsthoth hives, n: 10 were control and others 34 seek queen and placed it in a split hive (SHQR) with 5 frames with brood,1 of honey and 1 of beeswax. Keep in the original hive (OHQL) 2 frames with capped brood one of beeswax and one with honey. The OHQL rotate the entrance in the opposite direction (180 degrees). A double mesh floor was placed between both hive bodies. 24 hours later SHQL put it down and relocated within the same apiary, put the queen cell. All the hives were 1 liters of sucrose syrup every 7 days. 18 days check 31of 34 SH had mated queen (91, 17%). 45 days: measured the amount of frames covered by bees OH 7, 59 NH 5, 77. The production of honey in kilograms during 2014 was OH 25.30 NH 22.5. Control: 23, 50. Winter losses OH 5,88% NH 2,94 % Control 10%. We can conclude in the conditions of this assay it is possible to perform the multiplication of hives with actual cells with high levels of efficiency and harvest honey in the following season. Support PRODEAR.

TQP-042

New controlled release delivery system for propolis

Konstantinos Gardikis1, Antonios Tsagkarakis2, Anagnosti-John Choukalas1, Eleni

Spanidi1, Panagiota Dragani1, Christina Papitsa1, Petros Bozidis3, Anna Goussia3, Athanasios

Karapetsas4, GeorgiaPersephoni Voulgaridou4, Aglaia Pappa4, Mihalis Panayiotidis5

450

1 APIVITA S.A., Greece

2 Institute of Agronomical Sciences, Greek Ministry of Rural Development and Food 3 Department of Pathology School of Health Sciences, University of Ioannina, Greece

4 Department of Molecular Biology & Genetics, Democritus University of Thrace, Alexandroupolis, Greece

5 School of Life Sciences, Heriot Watt University, Edinburgh, Scotland

Propolis is a resinous material produced by honey bees. It exhibits antimicrobial and antioxidant properties and is used in the preparation of foodstuffs and cosmetics. Its composition varies depending on the plant origin, the geographical location and the collection season, thus its standardized formulation is difficult. The active components of propolis are polyphenols, terpenes, steroids, as well as sugars and aminoacids. Due to the complexity of propolis structure, the extraction of propolis usually has several issues such as the low yield in polar solvents and the poor stability and bioavailability. In this study a new delivery system for the green extraction and simultaneous encapsulation of propolis’ polyphenols in natural carriers was developed. The new system is standardized in polyphenol content and exhibits in vitro controlled release properties. Its toxicity and bioactivity profile (antioxidant, anti-mutagenic and anti-ageing potential) was assessed against normal human epidermal keratinocytes under normal and UV exposure conditions. The new system is currently further evaluated for its biological properties on a reconstituted skin model.

TQP-043

The effect of different feedbee’s concentration level on the bee colonies’ development during the spring season

Sabah Sena, Martin Llazan, Lumturi Sena1, Anila Hoda

Ag. Uni. Tirana, Albania

To estimate the effect of use of different Feedbee’s concentration level in the development of bee colonies during

Spring Season, an experimental trial was carried out in an apiary in Tirana. For this trial, 21 bee colonies, similar towards strength and Queen’s age, divided into three groups, were selected. The First Group (G1), during the whole trial’s period, was fed with 4% Feedbee, the Second Group (G2) was fed with 10%, while the Third Group (G3) with 35%. The trial was organized into two phases, starting on January 20th through April 30th, each of them 45 days long. The feed consumption per each colony/phases was 2 kg. During both phases, the number of brooding frames and size of caped brood area in each group was measured. At the end of this trial, it was concluded that the application of different Feedbee’s concentration level has not demonstrated any effect on the number of brooding frames. The application of the 10–35% concentration level of the Feedbee during the First Phase has stimulated a higher brooding rate up to 52% more, while during the Second Phase this effect is not evident, since the differences among the groups are not significant (p < 0.05). Using the 10% Feedbee during the Phase 1, since the differences among the G2 vs G3 were not significant, 2. 42 Euro/bee-colony will be saved. During the Second Phase the least concentration can be effectively applied, saving 3.4 Euro/bee-colony.

TQP-044

Application of GIS in potential beekeeping assessment: Case study of Montesinho Natural Park (Portugal)

451

Miguel Vilas-Boas4, Paulo Fernandez1, Helder Garção2, Natália Roque3, Fátima Peres3,

Manuel Gonçalves2, Ofélia Anjos5

1 Instituto Politécnico de Castelo Branco, Escola Superior Agrária, Apartado 119, 6001-909 Castelo Branc o, Portugal; ICAAM - Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de É vora, Pólo da Mitra, Ap. 94, 7002-554 É vora, Portugal

2 Associação dos Apicultores do Parque Natural de Montesinho, Quinta Fontainhas. 5300-100 Bragança, Portugal

3 Instituto Politécnico de Castelo Branco, Escola Superior Agrária, Apartado 119, 6001-909 Castelo Branc o, Portugal

4 Mountain Research Centre (CIMO), Instituto Politécnico de Bragança, Campus de Sta. Apolónia, 5300-2 53 Bragança, Portugal

5 Instituto Politécnico de Castelo Branco, Escola Superior Agrária, Apartado 119, 6001-909 Castelo Branco, Portugal; Centro de Estudos Florestais, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal

Planners require solutions that address routine work needs and seems essential to improving efficiency and productivity. There are a great number of different factors related to beekeeper activity as well the quality and productivity of different bee products. The spatial analysis is a powerful tool for overlap and relates various levels of information on a map, and consequently a very useful for beekeeping activity planning. This work proposes and applies a methodology to potential beekeeping assessment in Montesinho Natural Park, a region in the northwest of Portugal. The beekeeping potential maps were developed with the following data sources: legal standards, vegetation, land use, topography, water resources, roads, electromagnetic fields, and some honey physico-chemical analysis. The design and implementation of spatial analysis model based on Geographic Information System (GIS) to beekeeping planning activities has already been described by Anjos et al (2014). Spatial analysis techniques allows to define the potential beekeeper map supporting the beekeeper management in this region. Anjos O, Silva G, Roque N, Fernandez P, 2014. GIS based analysis to support the beekeeping planning. Book of abstracts of the International Symposium on Bee Products 3rd edition – Annual meeting of the International Honey Commission (IHC), Faculty of medicine, University of Rijeka, p:61.

TQP-045

Discovery of phytochemical biomarkers in propolis using 15t fourier transform ion cyclotron resonance mass spectrometry

Eunjung Son1, Narae Kim2, Myung-sang Kwon2, Mee Ree Kim3, Hyun Sik Kim4

1 KBSI, CNU

2 Seoul Propolis

3 CNU

4 KBSI, Republic of Korea

Propolis is a resin that bees collect from different plant sources and use for the defence of bee community. The intricate composition of propolis varies depending on plant sources from different geographical region and growing environment. Propolis is being widely used as dietary supplements, cosmetics, and toothpaste based on its anti-microbial and antioxidant activities but the activities have a wide fluctuation depending on the

452

compositions and quantities. To produce the constant efficacy, propolis needs to be thoroughly analysed to find its markers and index compounds and then be standardized by chemical compositions of the raw material. We investigated 11 different geographical samples collected from China, Korea, Argentina, Brazil (green and brown), Malaysia, Chile, India, Kyrgyzstan, and Uruguay. Constituent chemicals were extracted from the samples with 70% aqueous ethanol and then investigated by LC-MS and direct infusion MS on an electrospray ionization Fourier transform ion cyclotron resonance mass spectrometer (ESI FT-ICR MS) and a quadrupole time-of-flight mass spectrometer (Q-ToF MS). Chemical fingerprints were analysed by principal component analysis (PCA) and hierarchical cluster analysis (HCA) according to their growing environments. Despite their similar appearance such as colour and smell, the propolis samples were classified into different groups due to their characteristic chemical compositions. The result has shown that the high resolution mass profile can be a very specific method to distinguish the propolis samples by their growing areas and identify the characteristic marker compounds for their medicinal activities.

TQP-046

Using tics in the evaluation of prototypes of hives for Apis mellifera adapted to high temperatures in Latin America and Caribbean

Emilio Figini7, Emanuel Caluva1, Ezequiel Cecotti2, Juan Dukart3, Andres Moltoni4, Javier

Caporgno5, Cecilia Dini6

1 INTA - Becario Apicultura de Precisión - EEA Concepción del Uruguay. caluva.emanuel@inta.gob.ar 2 Promotor Asesor Cambio Rural

3 Responsable del Programa Apícola de la Asociación para el Desarrollo Regional de Ceres y Hersilia. 4 INTA - Responsable Laboratorio de Electrónica - Instituto de Ingeniería Rural - Castelar

5 INTA - Coordinador del PNAPI 1112051)

6 INTA - Coordinador del Proyecto REDLAC (PNAPI 1112053) 7 EEA-INTA BALCARCE-Buenos Aires-Argentina-PROAPI

One of the consequences of climate change (CC) in Latin America and Caribbean (LAC) is the increase in the occurrence of extreme events. In recent years, extremely high temperatures have caused absconding of colonies and melting of thousands hives in this region, where Langstroth´s hives, developed for temperate climates in 1852, is mostly used. With the aim of improving the conditions of comfort for the colonies of Apis mellifera without altering the size of the standart material used, a trial was carried out in an apiary located in province of Santa Fe (29 ° 54 '52.17 "lat. S, 61 ° 54 '40.81 " long). Eight prototypes were evaluated, all of them designed according the contributions of technicians and beekeepers of affected regions, using two treatments: with and without bees in its interior. Two dataloggers designed in the Electronics laboratory of the IIR (INTA Castelar) were used with 8 analog temperature (TMP36) and 5 digital sensors of temperature and humidity (SHT11) each. SD memory card reader, they have to store the recorded data, at 10 minute intervals, in ".csv" formatted files. The dataloggers are powered sealed battery 12V 7Ah connected to a solar panel. In this exploratory stage, the designs of best perfomance were the prototypes which reduce the external input of air and have any insulation on the top. These prototypes will be evaluated in apiaries with a higher number of repetitions in a second stage. Support: FONTAGRO FTG/FR 1331/RGPNAPI 1112053

453

Apitherapy

ATP-001

Therapeutic effect of propolis on alpha amanitine toxicity

Meral Kekeçolu1, P GOÇ Rasgele1, R Bayram2, E Kaya1, M Kambur1

1 Düzce University

2 Abant zzet Baysal University

Intoxications caused by -AMA containing mushrooms represent an unresolved problem in clinical toxicology since no specific and fully efficient antidote is available.Therefore, mushroom intoxication and its treatment are major research subjects. In the present study, it was aimed to determine therapeutic effects of propolis extracts in different solvents such as DMSO, water, ethanol, L-lysine against to the -AMA toxicity on C3A cell line. MTT assay were performed to evaluate the cell viability of hepatocyte C3A cells following propolis treatment and compared with cells treated silibinin which commonly used as an antidote of -AMA. In cultures treated with four different extracts of propolis without -AMA, cell viability significantly increased with only ethanolic extract of propolis in a dose-dependent manner. The C3A cell lines including -AMA (5 g/ml) treated with propolis (0.5,

1, 5 and 10 g/ml), and silibinin (0.1, 1, 10 and 100 m) each of 48h to investigate conventional method to new and natural remedy. After cell culture infused with -AMA and treated various propolis extracts and silibinin, results showed that the most efficient propolis extract was aquatic extract of propolis (AEP). The efficacy of aquatic extract of propolis (AEP ) at 10 g/ml and the efficacy of silibinin (10 µm) indicated the same rate therapeutic effect against the toxicity of -AMA in cell culture. Additionaly, aquatic propolis (10 g/ml) extraction has revealed higher antitoxic effect against to -AMA in vitro as compared to alcohol extraction (10 g/ml).

Acknowledgment:This study was supported by TUBITAK, ARDEB-3001, Project No:114S024

ATP-002

Antibacterial activity of royal jelly (Apis mellifera) proteins

Josely Borges, Elizabeth Ortiz-Vazquez, Ezequiel Lopez-Baños, Saul Tejera-Perez,

Jesus Ramon-Sierra, Jorge RuizRuiz

Instituto Tecnologico de Merida, Mexico

Royal jelly is a natural and biodegradable product and several reports have validated the relationship between continuous consumption of royal jelly and health. Several studies have demonstrated the potential of this natural product as an antioxidant, neur otrop hic , hyp o glycemic , hyp o cho lester olemic , hep atopro tective , hyp otensive , anti -tumo r , anti -inflammato r y, inmunomodulatory and anti-allergic. The aim of this study was to determine the properties of proteins of royal jelly and evaluate its antibacterial activity. Royal jelly samples were collected directly from hives in the state of Yucatan, Mexico. Strains used were E. faecalis ATCC 29212, E. coli ATCC 25922, S. aureus ATCC 25923 and V. cholerae. Protein extract was purified by sizeexclusion chromatography (Sepharose-G200). The antibacterial effect of purified proteins was tested by disk diffusion method. Proteins were analyzed by SDS-PAGE (Bizan i et al., 2005). Proteolytic activity was determined using substrate-gel electrophoresis (Abreu-Payrol et al., 2005). A total of 46 fractions were obtained by size-exclusion chromatography from the royal jelly protein extract. The SDS-PAGE gel of the group of fractions called RJP2 showed four bands with molecular weights of 32, 45, 55, and 62 kDa. RJP2 showed proteolytic activity and antimicrobial activity against the strains tested, with inhibition diameters of 1.10 cm (E. coli), 2.20 (E. aureus),

454

1.24 cm (V. cholerae), and 1.66 cm (E. faecalis). The protein extract of ro yal jelly and RJP2 fraction showed proteolytic activity and wide spectrum of antibacterial activity against gram positive and gram negative microorganism. The results indicate that proteases could contribute significantly in the antibacterial activity of royal jell y.

ATP-003

Comparing the antioxidant potentials of honey, propolis and locust and developing their healthy mixture

Asli Elif Sunay1, Aynur Ç etin1, Oykum Bahar Esen2, Dilek Boyacolu3, Taylan Samanc1, Mustafa Bayraktar1

1 SBS-Scientific Bio Solutions Turkey LLC, Maslak, Istanbul – Turkey

2 Department of Food Engineering, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey

3 SBS Scientific Bio Solutions, LLC, ITU Campus, Maslak, stanbul, Turkey, Department of Food Engineeri ng, Istanbul Technical University, 34469, Maslak, Istanbul, Turkey

Among bee products, honey and, especially, propolis exhibit greater antioxidant capacity, providing the favourable effects on human health. The carob pod’s high polyphenolic content and its strong antioxidant activity make this nutrient to be a potential candidate for developing a healthy food. The aim of this study was to assess the total phenolic and flavonoid contents of honey, propolis, locust samples and to develop a healthy new food product. Total contents of phenolics (TCP) and flavonoids (TFC) as well as total antioxidant capacities were measured by Folin-Ciocalteu and AlCl3 coloration methods, and by CUPRAC (cupric reducing antioxidant capacity) methods, respectively. The results indicated that honey (0.5 gGAE/g) gave the lowest responses for TPC, while the responses of propolis (199.1 gGAE/g) were highest. Also, the highest values were obtained in ethanolic extract of propolis (7915.2 gCatechin/100g). The order for antioxidant capacity of honey, propolis and locust was found to be as: propolis (354.0 gTEAC/g) > locust (19.8 gTEAC/g) > honey (1.1 gTEAC/g). The mixture developed by the proper addition of each ingredient was evaluated by conducting a sensory panel focusing on consumer likings. A total of 144 untrained panelists assessed their preference of liking for one sample pair using 2-Alternative Forced-Choice test. Friedman test of rank sums of each batch revealed that the samples containing the Carob pod were liked over other treatments (p0,05).

ATP-004

Inhibition of Candida albicans biofilm through Bifidobacteria isolated from the digestive tract of Saudi Arabian honeybee race, Apis mellifera jemenitica

Mohammad javed Ansari, Ahmad Al-Ghamdi, Khalid Khan, Adgaba Nuru, Noori Al-Waili

Chair of Eng. Abdullah Baqshan for Bee Research, Department of Plant Protection, College of food and A gricultural sciences, King Saud University Riyadh, Kingdom of Saudi Arabia, Saudi Arabia

We investigated the effect of probiotic Bifodobacterium (ksuBF4), isolated from the gut of A. m. jemenitica, on biofilm formation of C. albicans. The ability of C. albicans to adhere and grow on a polystyrene plates, reflecting

455

biofilm formation, was studied in the presence of Bifidobacterium. The effect of Biofidobacterium culture supernatant on the viability of C. albicans was studied as well

ATP-005

Bioactive qualifications and characteristics of cherry honey,

bee pollen and bee bread

Muhsin Dogaroglu4, Sevgi Kolayl1, Banu Yücel2, Oktay Yldz Hilal Hotaman3, Erkan Topal

Mustafa Kosoglu5

1 Karadeniz Technical University, Faculty of Science, Department of Chemistry, Trabzon/Turkey 2 Ege University Faculty of Agriculture, Department of Animal Science, Izmir/Turkey

3 Karadeniz Technical University, Macka Vocational High School, Department of Food Processing, Trabzo n/Turkey

4 Doga Beekeeping Company, Tekirdag/Turkey

5 Ministry of Food, Agriculture and Livestock, Aegean Agricultural Research Institute, Department of Beek eeping, Izmir/Turkey

In this research, physicochemical properties and active biological components of cherry honey, bee pollen and bee bread have been researched for moisture, conductivity, hunter color, optic rotation, total protein, prolin, phenolic content, flavonoids, antioxidant capacity, DPPH radical scavenging activity, anti-hyaluronidase activity. According to results, prolin has found 633 mg/kg in cherry honey. Total phenolic was 1371 mg/100g in bee pollen and 1428 mg/100g in bee bread whereas total antioxidant capacity (FRAP) was 5908 mgFeSO4/100 gand 4634 mgFeSO4/100 g, respectively. Cherry honey has characteristics with light color (L:80)in whitish-yellow. Antihyaluronidase activity of cherry honey (0.55 IC50 (g/mL)wa s found higher than bee pollen (0.02 IC50 (g/mL)and bee bread (0.01 IC50 (g/mL). Cherry pollen and bee bread have high antioxidant and anti-inflammatuar capacity due to their excessively having phenolic components. As a result, cherry honey, pollen and bee bread have high theraperutic specifications and more intensive research should be done for usage of them in Apitherapy.

Key Words: Cherry honey, pollen, bee bread, apitherapy

ATP-006

The inhibitory effects of bee venom on atopic dermatitis in an animal model

Hyun Jin An, Jung Yeon Kim, Woon Hae Kim, Kwan Kyu Park, Hyun Chung

Republic of Korea

Atopic dermatitis (AD) is a multifactorial skin disease, with complex interactions of innate and adaptive immune responses based on a genetic, pharmacological and psychological predisposition. Purified Bee venom (BV) is a natural toxin produced by honeybees. BV has been widely used as a traditional medicine for various diseases. However, the precise mechanism of BV in ameliorating the AD is not fully understood. We investigated the pharmacological effects of BV on 1-Chloro-2,4-dinitrobenzene (DNCB)-induced AD in mice. BALB/c mice were sensitized with 200 µl of 1% DNCB in acetone-olive oil (3:1) applied to the dorsal skin twice per week for

456

2 weeks. And then, the treatment of 0.2% DNCB was repeated twice a week alternatively for 4 weeks. BV (100, 200 µg in mixed with Placebo) was applied to the dorsal skin. Treatment with BV inhibited the eosinophil and mast cell infiltration into AD skin tissue. In addition, BV attenuated the production of histamine and proinflammatory cytokines in AD mice. The finding of this study demonstrated that BV reduced DNCB-induced AD in mice. Collectively, this study provides us with novel insights into the pharmacological actions of BV as a potential molecule for use in the treatment of allergic inflammation diseases.

ATP-007

Determination of flavonoid classes in Trigona sp. propolis from Luwu Regency, South Sulawesi Province, Indonesia

Ardo Sabir1, Latief Mooduto2, Cahyono Kaelan3, Sherly Horax4

1 Indonesia Dentist Association

2 Faculty of Dentistry, Airlangga University

3 Faculty of Medicine, Hasanuddin University

4 Faculty of Dentistry, Hasanuddin University, Indonesia

Propolis is a resinous hive product, collected by bees. Recently, propolis has been used clinically by dentist to treat various oral diseases such as to support aid for the healing of post-extraction wounds, as an antimicrobial agent in the treatment of dental caries and pulpal infections, and to promote dentinal bridge formation in direct pulp-capping treatment. Propolis contains waxes, ferulic acid, cinnamic acid, phenol acids, aromatic aldehydes, and a large number of flavonoids. The application of propolis preparations in dentistry leads to increase interest in its chemical composition.

The aim of this research was to determine flavonoid classes in Trigona sp propolis which was collected from honeycombs in Luwu regency, South Sulawesi Province, Indonesia. Propolis was subjected to exhaustive maseration, filtered using aqueous ethanol solution and concentrated using a rotary evaporator. The residue was separated using toluene solution to yield polar flavonoid fraction and semi-polar flavonoid fraction which was analyzed using Thin Layer Chromatography (TLC) method (silica gel GF254 precoated plates with n-butanol: acetic acid: water (3: 1: 1 v/v) as mobile phase). The purification of flavonoids was carried-out by UV-radiation at 253 nm and 366 nm and treatment with ammonia. The idenfication flavonoid classes based on color reactions according Mabry et al.

The result of this research showed that the flavonoid classes in the Trigona sp propolis from Luwu regency, South Sulawesi province, Indonesia were flavones, flavanone, flavonol, and chalcone classes.

ATP-008

Beneficial properties of argentine stingless bees propolis: Anitnociceptive, antiinflammatory and anti-pathogenic activities

Emilio Figini4, Ivana Brodkiewicz1, Gerardo Gennari2, Luis Maldonado3, Nancy Vera5

1 Universidad Nacional de Tucumán (UNT). Ayacucho 471, 4000, Tucumán.veranr@gmail.com

2 Instituto Nacional de Tecnología Agropecuaria (INTA).PROAPI. Famaillá, 4132, Tucumán. Argentina.

457

3 Instituto Nacional de Tecnología Agropecuaria (INTA). PROAPI. Famaillá, 4132, Tucumán. Argentina. 4 INTA PROAPI. Fac. de Cs. Veterinarias (UNCPBA) -Tandil-Argentina

5 Universidad Nacional de Tucumán (UNT). Ayacucho 471, 4000, Tucumán. INTA PROAPI

Propolis from stingless bees is well known for its biologic properties; however, few studies have demonstrated these effects. Therefore, this study aimed to investigate the antinociceptive, anti-inflamatory and anti-biofilm activities of alcoholic extracts propolis from the stingless bees Tetragonisca fiebrigi y Scaptotrigona jujuyensis, produced in Estación Experimental Agropecuaria Famaillá del INTA, Tucumán, Argentina. Anti-inflammatory activity was evaluated using carrageenan induced paw oedema. Analgesic activity of the extracts was estimated against acetic acid induced writhing, tail immersion method, formalin test. The anti-biofilm activity of these propolis was tested on Pseudomonas aeruginosa and Staphylococcus aureus bacterias. Both propolis extracts induced a significant anti-inflammatory effect, reaching maximum inhibition (55% T. fiebrigi and S. jujuyensis 62% respectively) at 1 hour with the highest dose, similar to ibuprofen (100%). It showed that alcoholic extracts of propolis studied species possess antinociceptive activity at both central and peripheral levels demonstrated by the three test algesia used. Scaptotrigona jujuyensis propolis tested at doses of 200 and 100 ug/ml against Pseudomonas aeruginosa presented a selective inhibition of biofilm unrelated to inhibition of bacterial growth, thus achieving reduce pathogenicity. It has started the isolation and identification of compounds responsible for the pharmacological activities displayed by propolis.

ATP-009

Bee bread: Origin, Composition, Properties and Uses against aluminium chloride-induced anemia in wistar rats

Badiaa Lyoussi1, Meryem Bakour2, Nawal El Menyiy2, Hamada imtara2

1University USMBA, Fez

2University USMBA, Fez

Aluminum (Al) toxicity was associated with hypochromic microcytic anemia through observations of patients undergoing renal dialysis and Al-containing phosphate binder treatment. Bee bread is a mixture of pollen loads, honey milk enzymes and various lactobacilli that serves basic food for bees. Due to its more complex composition and to its easier digestibility/bioavailability, bee bread is valuable in the treatment of severe diseases like cardiovascular pathologies, diabetes. The present studies were undertaken to assess the effect of chronic administration of aluminum (AlCl 3,6H2O (DL10 = 662.2 mg/kg BW) on the perturbation of the haematological parameters and the production of a hypochromic microcystic anemia in Wistar rats, and to study the protective effect of co-administration of the extract of bread bee and aluminum. The measured parameters are : hematocrit % ; red blood cells (RBC); platelets ; hemoglobin (Hb); lymphocytes ; monocytes; mean corpuscular volume (MCV) ; mean corpuscular hemoglobin concentration (MCHC); Red blood cell distribution width (RDW) ; mean platelet volume (MPV) ; leucocytes (wbc); sodium ; potassium ; urea ; renal clearance. The results of this study shows the protective effect of bee bread extract on anemia induced by aluminum in rats. The major finding of this study is that aluminum enhanced oxidative stress. Bee bread showed a significant improvement in reduction of this oxidative stress by enhancement of superoxide dismutase and catalase levels. This effect may be attributed mainly to the active components present in bee bread.

458

ATP-010

In vitro screening for Anti-inflammatory activity of Moroccan propolis

Badiaa Lyoussi1, Soukaina El-Guendouz1, Smail Aazza1, Maria da Graça Miguel2

1 University USMBA, Fez, Morocco

2 Faculdade de Ciências e Tecnologia, Faro, Portugal,

Propolis is a natural substance, produced by honeybees from the gum of various plants. Propolis chemical composition has been correlated with plant diversity around the beehive, It contains several chemical compounds, including various phenolic compounds like flavonoids (galangin, quercetin), cinnamic acid and its derivatives (chlorogenic acid, ferrulic acid, caffeic acid phenethyl ester), various steroids, amino acids and volatile aldehydes and ketones[1 ].

The Capacity to inhibit 5-lipoxygenase and hyaluronidase of 24 propolis samples collected from various regions of Morocco were evaluated in this work.

A lipoxygenase assay was used as an indicator of anti-inflammatory and antioxidant activities. aqueous ethanol extracts of propolis were revealed to have different inhibition acitivities with IC50 values ranging from from 0.02 to 0.65mg/ml. Sample S1 showed the lowest IC50 values (0.02 mg/ml), this sample was more effective than nordihydroguaiaretic acid (IC50=0.02 mg/ml). The highest hyaluronidase inhibition was recorded in the sample S1 with 92.3 + 0.7%, Folowed by sample S10 with 87.86 + 0.6% and Sample S22 with 87.04 + 0.1%, while le sample S6, showed le lowest inhibition wit 7.6 + 0.1%. Keywords: lipoxygenase, Propolis, Hyaluronidase. [1 ] W. Greenaway, T. Scaysbrook, F.R. Whatley (1988) Composition of propolis in Oxfordshire, U. K. and its relation to poplar bud exudates. Z Naturforsch, 43, p. 301

ATP-011

Tyrosinase and xanthine oxidase inhibitory activities of Moroccan propolis

Badiaa Lyoussi1, Soukaina El-Guendouz1, 1Smail Aazza1, Maria da Graça Miguel2

1 Laboratory of Physiology, Pharmacology and Environmental Health, University Sidi Mohamed Ben Abdal lah, Fez, Morocco

2 2Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian 2Faculdade de Ciências e Tec nologia, Edif. 8, Campus de Gambelas, 8005-139 Faro, Portugal

Propolis is a colored and aromatic colloidal substance collected by honeybees through adding their saliva secreted to the resinous plant exudates, which is used to build honeycomb and to fight against the invasion of pathogenic microorganisms . It has been applied in popular folk medicine since 3000 BC due to possessing a broad spectrum of biological activities such as antioxidant, antimicrobial, anti-inflammatory, antiviral, anticancer, and antihepatotoxic. Tyrosinase and xanthine oxidase inhibitory activities of 24 propolis samples collected from various regions of Morocco were evaluated in this work. The effectiveness of enzymatic inhibition of the various extracts was determined by calculating IC50. The lower the value showed, the higher the enzymatic inhibition. Xanthine oxidase inhibitors are known to be therapeutically useful for the treatment of gout, hepatitis and brain tumor. Tyrosinase inhibitory activity is used for the treatment of hyperpigmentation and other related disorders. IC50 values of propolis extracts ranged from 0.037 to 4.28mg/ml against mushroomTyrosinase and from 0.007 to 3.12mg/mL against xanthine oxidase.

459