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122 Diagnostic Tests

Figure 3. Amplification plot of "TaqMan" (5'nuclease) real-time PCR for the detection of SARS-CoV RNA in clinical specimens. This is a widely used assay, developed by BNI. Primers and fluorescence-labeled probe are located in the polymerase gene of SARS-CoV (Picture source: Institute for Medical Virology, Director: W. Doerr).

References

1.Drosten C, Gunther S, Preiser W, et al. Identification of a Novel Coronavirus in Patients with Severe Acute Respiratory Syndrome. N Engl J Med 2003; 348:1967-76. Published online Apr 10, 2003. http://SARSReference.com/lit.php?id=12690091

2.Lai MM, Cavanagh D. The molecular biology of coronaviruses. Adv.Virus Res. 1997; 48:1-100.

3.Li G, Chen X, Xu A. Profile of specific antibodies to the SARSassociated coronavirus. N.Engl.J.Med. 2003; 349:508-509.

4.McIntosh K. The SARS coronavirus: rapid diagnostics in the limelight. Clin Chem 2003; 49: 845-6. http://SARSReference.com/lit.php?id=12765977

www.SARSreference.com

References 123

5.Peiris JS, Chu CM, Cheng VC, et al. Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study. Lancet 2003b; 361:1767-

72. Published online May 9, 2003. http://image.thelancet.com/extras/03art4432web.pdf

6.Poon LL, Wong OK, Luk W, Yuen KY, Peiris JS, Guan Y. Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). Clin Chem 2003; 49: 953-5. Erratum in: Clin Chem. 2003 Jul;49(7):1234. http://SARSReference.com/lit.php?id=12765993

7.WHO Update 71. Status of diagnostic tests, training course in China. http://www.who.int/entity/csr/don/2003_06_02a/en

8.WHO. Outbreak in British Columbia, Canada is not SARS. http://www.who.int/csr/don/2003_08_25a/en/

Kamps and Hoffmann (eds.)